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kinematic fluorescence filter cube  (Thorlabs)

 
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    Thorlabs kinematic fluorescence filter cube
    Kinematic Fluorescence Filter Cube, supplied by Thorlabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kinematic+fluorescence+filter+cube/fluorescence+cube+dfm1/pm39401353-205-36-40
    Average 90 stars, based on 1 article reviews
    kinematic fluorescence filter cube - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Somatostatin peptide signaling dampens cortical circuits and promotes exploratory behavior
    Article Snippet: .. Blue light from a 473-nm laser (MBL-III-473–100mW, Ready Lasers) was directed through a Noise Eater (NEL01, ThorLabs) and into a kinematic fluorescence filter cube (DFM1, ThorLabs) onto a dual-edge dichroic mirror (ZT488/561rpc, Chroma). .. Light was then coupled using an FC/PC fiber coupler (PAF2-A4A, ThorLabs) into a fiber patchcord (M72L05, 200-μm core, 0.39 NA, ThorLabs) connected to a fiberoptic rotary joint (FRJ_1×1_FC-FC, Doric Lenses) followed by another patchcord (200-μm core, 0.39 NA, Doric).

    Article Title: Hyperspectral oblique plane microscopy enables spontaneous, label-free imaging of biological dynamic processes in live animals.
    Article Snippet: The line illuminated by the laser is finally imaged horizontally (in the x’ direction) onto a camera (PCO.edge 5.5 m) with a total magnification of 33×. .. For the spectral resolution, a horizontal grating (ThorLabs GR25-0310, 300 lp/mm, 8.4° blaze angle, tilted by about 5°) together with a longpass filter (Semrock LP02-664RU) is inserted using a custom insert (SI Appendix) in a commercial kinematic fluorescence filter cube (ThorLabs DFM1/M) between O3 and the tube lens before a second camera (PCO.edge 5.5 m). ..

    Article Title: Non-genetic optical neuromodulation with silicon-based materials
    Article Snippet: Oxygen plasma etcher (Plasma Etch, PE-100LF) Spin coater (Laurell, WS-650) Mask aligner (EV Group, EVG 620) Reactive ion etcher (Oxford Instruments, Plasma Pro NGP80 ICP) .. Borosilicate patch pipettes (4-inch thin wall with 1.5-mm outer diameter (OD) and 1.12-mm inner diameter (ID), World Precision Instruments, cat. no. TW150F-4) Upright microscope (Olympus, BX61WI) 532 nm DPSS laser (Laserglow Technologies, LRS-0532-PFM-00300–05) Fiber port (FC/PC, f=11.0 mm, 350 – 700 nm, Ø1.80 mm waist, Thorlabs, cat. no. PAF-X-11-PC-A) Single mode patch cables (405 – 532 nm FC/PC, 2m, Thorlabs, cat. no. P1–405B-FC-2) FC/PC fiber adapter plate (with external SM1 thread, Thorlabs, cat. no. SM1FC) Cage Plate (SM1-threaded 30 mm, 0.35” thick, 2 retaining rings, 8–32 tap, Thorlabs, cat. no. CP02) Cage assembly rod (3” long, Ø6 mm, 4 pack, Thorlabs, cat. no. ER3-P4) Achromatic doublet lens (f=45 mm, Ø1”, SM1-threaded mount, ARC: 400–700 nm, Thorlabs, cat. no. AC254–045-A-ML) Neutral density filters (twelve station dual filter wheel for Ø1” filters with base assembly, 10 ND filters include, Thorlabs, cat. no. FW2AND) Right-angle kinematic mirror mount (with tapped cage rod holes, 30 mm cage system and SM1 compatible, 8–32 and 1⁄4”−20 mounting holes, Thorlabs, cat. no. KCB1) Ø1” Broadband dielectric mirror (400 – 750 nm Thorlabs, cat. no. BB1-E02) Lens tube spacer (SM1 without internal threads, 2.5” long, Thorlabs, cat. no. SM1S25) Kinematic fluorescence filter cube (30 mm cage compatible, left-turning, 1⁄4”−20 tapped holes, Thorlabs, cat. no. DFM1L) Dichroic filter (TRITC, reflection band = 525–556 nm, transmission band = 580–650 nm, Thorlabs, MD568) 530 nm LED (mounted, Thorlabs, cat. no. M530L3) Adjustable collimation adapter (with Ø25 mm lens, AR coating: 350 – 700 nm, Thorlabs, cat. no. SM1P25-A) Water-immersion lens (Olympus, UMPLFLN20X/W NA0.5) Micro-manipulator (Scientifica, PatchStar) Headstage (Axon Instruments, CV203BU) Amplifier (Axon Instruments, Axopatch 200B) Digitizer (Axon Instruments, Digidata 1550B) Automatic temperature controller (Warner Instrument, TC-344B) Micropipette puller (Sutter Instrument, P-97) Data acquisition software (Molecular Devices, pClamp) .. Glass bottom dishes (Cellvis, cat. no. D35–10-1-N or World Precision Instruments, cat. no. FD35–100) Glass Pasteur pipets (disposable 9” borosilicate glass, Thermo Fisher Scientific, cat. no. 13–678-20D) Forceps (Dumont #5, cat. no. RS-5035-Carbon) Glass flat bottom vial (20 mL disposable scintillation vial, cat. no. FS74504–30) 0.22 μm filter (Millex GP, cat. no. SL9P033RS) 10 mL syringe (LuerLock Norm-ject, cat. no. 4100-X00V0) 2”×2” cotton gauze sponges (Thermo Fisher Scientific, cat. no. 22–362-178) Cell culture incubator (Thermo Fisher Scientific, HERACell 150i) 37 °C shaker (Denville Scientific Inc., Incu-Shaker Mini) Alcohol burner (Hach, cat. no. 2087742) Centrifuge (Thermo Fisher Scientific, cat. no. 75004505)

    Article Title: Somatostatin peptide signaling dampens cortical circuits and promotes exploratory behavior.
    Article Snippet: .. Blue light from a 473-nm laser (MBL-III-473-100mW, Ready Lasers) was directed through a Noise Eater (NEL01, ThorLabs) and into a kinematic fluorescence filter cube (DFM1, ThorLabs) onto a dual-edge dichroic mirror (ZT488/561rpc, Chroma). .. Light was then coupled using an FC/PC fiber coupler (PAF2-A4A, ThorLabs) into a fiber patchcord (M72L05, 200-mm core, 0.39 NA, ThorLabs) connected to a fiberoptic rotary joint (FRJ_1x1_FC-FC, Doric Lenses) followed by another patchcord (200-mm core, 0.39 NA, Doric).

    Article Title: Portable Detection Platform For Point Of Care Diagnostics
    Article Snippet: .. In order to hold everything in the fluorescence setup we used the DFM1(Thorlabs) which is a kinematic Fluorescence Filter Cube. .. The cube holds both the filters as well as a dichroic mirror, DMLP490R (Thorlabs), that sits in the middle of the cube to allow for the excitation light to go to the sample while also preventing it from reaching the camera.

    Article Title: Brain-wide multi-fiber recording of neuronal activity in freely moving mice
    Article Snippet: .. Kinematic fluorescence filter cube , Thorlabs , Cat#DFM1. .. 495 nm dichroic beamsplitter , Semrock , Cat#FF495-Di03-25 × 36.

    Article Title: Hyperspectral oblique plane microscopy enables spontaneous, label-free imaging of biological dynamic processes in live animals
    Article Snippet: The line illuminated by the laser is finally imaged horizontally (in the x’ direction) onto a camera (PCO.edge 5.5 m) with a total magnification of 33 × . .. For the spectral resolution, a horizontal grating (ThorLabs GR25-0310, 300 lp/mm, 8.4° blaze angle, tilted by about 5°) together with a longpass filter (Semrock LP02-664RU) is inserted using a custom insert ( SI Appendix ) in a commercial kinematic fluorescence filter cube (ThorLabs DFM1/M) between O3 and the tube lens before a second camera (PCO.edge 5.5 m). ..

    Microscopy:

    Article Title: Non-genetic optical neuromodulation with silicon-based materials
    Article Snippet: Oxygen plasma etcher (Plasma Etch, PE-100LF) Spin coater (Laurell, WS-650) Mask aligner (EV Group, EVG 620) Reactive ion etcher (Oxford Instruments, Plasma Pro NGP80 ICP) .. Borosilicate patch pipettes (4-inch thin wall with 1.5-mm outer diameter (OD) and 1.12-mm inner diameter (ID), World Precision Instruments, cat. no. TW150F-4) Upright microscope (Olympus, BX61WI) 532 nm DPSS laser (Laserglow Technologies, LRS-0532-PFM-00300–05) Fiber port (FC/PC, f=11.0 mm, 350 – 700 nm, Ø1.80 mm waist, Thorlabs, cat. no. PAF-X-11-PC-A) Single mode patch cables (405 – 532 nm FC/PC, 2m, Thorlabs, cat. no. P1–405B-FC-2) FC/PC fiber adapter plate (with external SM1 thread, Thorlabs, cat. no. SM1FC) Cage Plate (SM1-threaded 30 mm, 0.35” thick, 2 retaining rings, 8–32 tap, Thorlabs, cat. no. CP02) Cage assembly rod (3” long, Ø6 mm, 4 pack, Thorlabs, cat. no. ER3-P4) Achromatic doublet lens (f=45 mm, Ø1”, SM1-threaded mount, ARC: 400–700 nm, Thorlabs, cat. no. AC254–045-A-ML) Neutral density filters (twelve station dual filter wheel for Ø1” filters with base assembly, 10 ND filters include, Thorlabs, cat. no. FW2AND) Right-angle kinematic mirror mount (with tapped cage rod holes, 30 mm cage system and SM1 compatible, 8–32 and 1⁄4”−20 mounting holes, Thorlabs, cat. no. KCB1) Ø1” Broadband dielectric mirror (400 – 750 nm Thorlabs, cat. no. BB1-E02) Lens tube spacer (SM1 without internal threads, 2.5” long, Thorlabs, cat. no. SM1S25) Kinematic fluorescence filter cube (30 mm cage compatible, left-turning, 1⁄4”−20 tapped holes, Thorlabs, cat. no. DFM1L) Dichroic filter (TRITC, reflection band = 525–556 nm, transmission band = 580–650 nm, Thorlabs, MD568) 530 nm LED (mounted, Thorlabs, cat. no. M530L3) Adjustable collimation adapter (with Ø25 mm lens, AR coating: 350 – 700 nm, Thorlabs, cat. no. SM1P25-A) Water-immersion lens (Olympus, UMPLFLN20X/W NA0.5) Micro-manipulator (Scientifica, PatchStar) Headstage (Axon Instruments, CV203BU) Amplifier (Axon Instruments, Axopatch 200B) Digitizer (Axon Instruments, Digidata 1550B) Automatic temperature controller (Warner Instrument, TC-344B) Micropipette puller (Sutter Instrument, P-97) Data acquisition software (Molecular Devices, pClamp) .. Glass bottom dishes (Cellvis, cat. no. D35–10-1-N or World Precision Instruments, cat. no. FD35–100) Glass Pasteur pipets (disposable 9” borosilicate glass, Thermo Fisher Scientific, cat. no. 13–678-20D) Forceps (Dumont #5, cat. no. RS-5035-Carbon) Glass flat bottom vial (20 mL disposable scintillation vial, cat. no. FS74504–30) 0.22 μm filter (Millex GP, cat. no. SL9P033RS) 10 mL syringe (LuerLock Norm-ject, cat. no. 4100-X00V0) 2”×2” cotton gauze sponges (Thermo Fisher Scientific, cat. no. 22–362-178) Cell culture incubator (Thermo Fisher Scientific, HERACell 150i) 37 °C shaker (Denville Scientific Inc., Incu-Shaker Mini) Alcohol burner (Hach, cat. no. 2087742) Centrifuge (Thermo Fisher Scientific, cat. no. 75004505)

    Transmission Assay:

    Article Title: Non-genetic optical neuromodulation with silicon-based materials
    Article Snippet: Oxygen plasma etcher (Plasma Etch, PE-100LF) Spin coater (Laurell, WS-650) Mask aligner (EV Group, EVG 620) Reactive ion etcher (Oxford Instruments, Plasma Pro NGP80 ICP) .. Borosilicate patch pipettes (4-inch thin wall with 1.5-mm outer diameter (OD) and 1.12-mm inner diameter (ID), World Precision Instruments, cat. no. TW150F-4) Upright microscope (Olympus, BX61WI) 532 nm DPSS laser (Laserglow Technologies, LRS-0532-PFM-00300–05) Fiber port (FC/PC, f=11.0 mm, 350 – 700 nm, Ø1.80 mm waist, Thorlabs, cat. no. PAF-X-11-PC-A) Single mode patch cables (405 – 532 nm FC/PC, 2m, Thorlabs, cat. no. P1–405B-FC-2) FC/PC fiber adapter plate (with external SM1 thread, Thorlabs, cat. no. SM1FC) Cage Plate (SM1-threaded 30 mm, 0.35” thick, 2 retaining rings, 8–32 tap, Thorlabs, cat. no. CP02) Cage assembly rod (3” long, Ø6 mm, 4 pack, Thorlabs, cat. no. ER3-P4) Achromatic doublet lens (f=45 mm, Ø1”, SM1-threaded mount, ARC: 400–700 nm, Thorlabs, cat. no. AC254–045-A-ML) Neutral density filters (twelve station dual filter wheel for Ø1” filters with base assembly, 10 ND filters include, Thorlabs, cat. no. FW2AND) Right-angle kinematic mirror mount (with tapped cage rod holes, 30 mm cage system and SM1 compatible, 8–32 and 1⁄4”−20 mounting holes, Thorlabs, cat. no. KCB1) Ø1” Broadband dielectric mirror (400 – 750 nm Thorlabs, cat. no. BB1-E02) Lens tube spacer (SM1 without internal threads, 2.5” long, Thorlabs, cat. no. SM1S25) Kinematic fluorescence filter cube (30 mm cage compatible, left-turning, 1⁄4”−20 tapped holes, Thorlabs, cat. no. DFM1L) Dichroic filter (TRITC, reflection band = 525–556 nm, transmission band = 580–650 nm, Thorlabs, MD568) 530 nm LED (mounted, Thorlabs, cat. no. M530L3) Adjustable collimation adapter (with Ø25 mm lens, AR coating: 350 – 700 nm, Thorlabs, cat. no. SM1P25-A) Water-immersion lens (Olympus, UMPLFLN20X/W NA0.5) Micro-manipulator (Scientifica, PatchStar) Headstage (Axon Instruments, CV203BU) Amplifier (Axon Instruments, Axopatch 200B) Digitizer (Axon Instruments, Digidata 1550B) Automatic temperature controller (Warner Instrument, TC-344B) Micropipette puller (Sutter Instrument, P-97) Data acquisition software (Molecular Devices, pClamp) .. Glass bottom dishes (Cellvis, cat. no. D35–10-1-N or World Precision Instruments, cat. no. FD35–100) Glass Pasteur pipets (disposable 9” borosilicate glass, Thermo Fisher Scientific, cat. no. 13–678-20D) Forceps (Dumont #5, cat. no. RS-5035-Carbon) Glass flat bottom vial (20 mL disposable scintillation vial, cat. no. FS74504–30) 0.22 μm filter (Millex GP, cat. no. SL9P033RS) 10 mL syringe (LuerLock Norm-ject, cat. no. 4100-X00V0) 2”×2” cotton gauze sponges (Thermo Fisher Scientific, cat. no. 22–362-178) Cell culture incubator (Thermo Fisher Scientific, HERACell 150i) 37 °C shaker (Denville Scientific Inc., Incu-Shaker Mini) Alcohol burner (Hach, cat. no. 2087742) Centrifuge (Thermo Fisher Scientific, cat. no. 75004505)

    Software:

    Article Title: Non-genetic optical neuromodulation with silicon-based materials
    Article Snippet: Oxygen plasma etcher (Plasma Etch, PE-100LF) Spin coater (Laurell, WS-650) Mask aligner (EV Group, EVG 620) Reactive ion etcher (Oxford Instruments, Plasma Pro NGP80 ICP) .. Borosilicate patch pipettes (4-inch thin wall with 1.5-mm outer diameter (OD) and 1.12-mm inner diameter (ID), World Precision Instruments, cat. no. TW150F-4) Upright microscope (Olympus, BX61WI) 532 nm DPSS laser (Laserglow Technologies, LRS-0532-PFM-00300–05) Fiber port (FC/PC, f=11.0 mm, 350 – 700 nm, Ø1.80 mm waist, Thorlabs, cat. no. PAF-X-11-PC-A) Single mode patch cables (405 – 532 nm FC/PC, 2m, Thorlabs, cat. no. P1–405B-FC-2) FC/PC fiber adapter plate (with external SM1 thread, Thorlabs, cat. no. SM1FC) Cage Plate (SM1-threaded 30 mm, 0.35” thick, 2 retaining rings, 8–32 tap, Thorlabs, cat. no. CP02) Cage assembly rod (3” long, Ø6 mm, 4 pack, Thorlabs, cat. no. ER3-P4) Achromatic doublet lens (f=45 mm, Ø1”, SM1-threaded mount, ARC: 400–700 nm, Thorlabs, cat. no. AC254–045-A-ML) Neutral density filters (twelve station dual filter wheel for Ø1” filters with base assembly, 10 ND filters include, Thorlabs, cat. no. FW2AND) Right-angle kinematic mirror mount (with tapped cage rod holes, 30 mm cage system and SM1 compatible, 8–32 and 1⁄4”−20 mounting holes, Thorlabs, cat. no. KCB1) Ø1” Broadband dielectric mirror (400 – 750 nm Thorlabs, cat. no. BB1-E02) Lens tube spacer (SM1 without internal threads, 2.5” long, Thorlabs, cat. no. SM1S25) Kinematic fluorescence filter cube (30 mm cage compatible, left-turning, 1⁄4”−20 tapped holes, Thorlabs, cat. no. DFM1L) Dichroic filter (TRITC, reflection band = 525–556 nm, transmission band = 580–650 nm, Thorlabs, MD568) 530 nm LED (mounted, Thorlabs, cat. no. M530L3) Adjustable collimation adapter (with Ø25 mm lens, AR coating: 350 – 700 nm, Thorlabs, cat. no. SM1P25-A) Water-immersion lens (Olympus, UMPLFLN20X/W NA0.5) Micro-manipulator (Scientifica, PatchStar) Headstage (Axon Instruments, CV203BU) Amplifier (Axon Instruments, Axopatch 200B) Digitizer (Axon Instruments, Digidata 1550B) Automatic temperature controller (Warner Instrument, TC-344B) Micropipette puller (Sutter Instrument, P-97) Data acquisition software (Molecular Devices, pClamp) .. Glass bottom dishes (Cellvis, cat. no. D35–10-1-N or World Precision Instruments, cat. no. FD35–100) Glass Pasteur pipets (disposable 9” borosilicate glass, Thermo Fisher Scientific, cat. no. 13–678-20D) Forceps (Dumont #5, cat. no. RS-5035-Carbon) Glass flat bottom vial (20 mL disposable scintillation vial, cat. no. FS74504–30) 0.22 μm filter (Millex GP, cat. no. SL9P033RS) 10 mL syringe (LuerLock Norm-ject, cat. no. 4100-X00V0) 2”×2” cotton gauze sponges (Thermo Fisher Scientific, cat. no. 22–362-178) Cell culture incubator (Thermo Fisher Scientific, HERACell 150i) 37 °C shaker (Denville Scientific Inc., Incu-Shaker Mini) Alcohol burner (Hach, cat. no. 2087742) Centrifuge (Thermo Fisher Scientific, cat. no. 75004505)

    other:

    Article Title: Pan-cortical 2-photon mesoscopic imaging and neurobehavioral alignment in awake, behaving mice
    Article Snippet: Eyoyo 15.6" inch Gaming Monitor 1920x1080 HDR Display Second (newegg.com) E-650 Piezo Amplifier for Multilayer bending actuators, 18 W PICMA® multilayer piezo bending actuator, 2000 μm travel range, 45 mm × 11.00 mm × 0.55 mm, stranded wires (PL140.11; physik instrumente.store) Tucker Davis ES1 Free Field Electrostatic Speaker Tucker Davis ED1 Electrostatic Speaker Driver Oben BD-0 mini ball head (amazon.com) Locking ball and socket mount, 1⁄4”-20 threaded (TRB2; Thorlabs) Mounted LED 470 nm (760 mW, 1000 mA) (M470L4, Thorlabs) Dichroic (T495lpxr, chroma.com) Excitation filter (ET470/40x; chroma.com) Emission filter (ET525/50m; chroma.com) Kinematic fluorescence filter cube (DFM1, Thorlabs) ScanImage 2p acquisition Spectra Physics Mai Tai HP-244 tunable femtosecond laser https://www.spectra-physics.com/en/f/mai-tai-ultrafast-laser Thorlabs Mesoscope https://www.thorlabs.com/newgrouppage9.cfm?objectgroup_id=10646 ScanImage 2018-2021 (Vidrio Technologies) https://vidriotechnologies.com/scanimage/ http://scanimage.vidriotechnologies.com/display/SIH/ScanImage+Home



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    Image Search Results


    Step-by-step assembly of the multi-fiber photometry recording system (part I) (A and B) Connect the power supply, LED driver, LED, and SMA-SMA patch cord. (C‒E) Attach the multimode collimator to an XY translator. (F‒J) Install the fluorescence filters into the fluorescence filter cube. (K and L) Attach the 10x objective to the fluorescence filter cube. (M‒Q) Attach the SMA fiber adapter plate to the fluorescence filter cube.

    Journal: STAR Protocols

    Article Title: Brain-wide multi-fiber recording of neuronal activity in freely moving mice

    doi: 10.1016/j.xpro.2024.102882

    Figure Lengend Snippet: Step-by-step assembly of the multi-fiber photometry recording system (part I) (A and B) Connect the power supply, LED driver, LED, and SMA-SMA patch cord. (C‒E) Attach the multimode collimator to an XY translator. (F‒J) Install the fluorescence filters into the fluorescence filter cube. (K and L) Attach the 10x objective to the fluorescence filter cube. (M‒Q) Attach the SMA fiber adapter plate to the fluorescence filter cube.

    Article Snippet: Remove the insert of the kinematic fluorescence filter cube (DFM1, Thorlabs) and disassemble it using a hex key ( F and 3G).

    Techniques: Fluorescence

    Step-by-step assembly of the multi-fiber photometry recording system (part II) (A‒D) Connect the Basler camera with a 30 mm cage plate. (E‒I) Attach the camera to the XY translator. (J‒M) Connect two XY translators with the fluorescence filter cube. (N) Attach the SMA-SMA fiber patch cable to the multimode collimator.

    Journal: STAR Protocols

    Article Title: Brain-wide multi-fiber recording of neuronal activity in freely moving mice

    doi: 10.1016/j.xpro.2024.102882

    Figure Lengend Snippet: Step-by-step assembly of the multi-fiber photometry recording system (part II) (A‒D) Connect the Basler camera with a 30 mm cage plate. (E‒I) Attach the camera to the XY translator. (J‒M) Connect two XY translators with the fluorescence filter cube. (N) Attach the SMA-SMA fiber patch cable to the multimode collimator.

    Article Snippet: Remove the insert of the kinematic fluorescence filter cube (DFM1, Thorlabs) and disassemble it using a hex key ( F and 3G).

    Techniques: Fluorescence

    Journal: STAR Protocols

    Article Title: Brain-wide multi-fiber recording of neuronal activity in freely moving mice

    doi: 10.1016/j.xpro.2024.102882

    Figure Lengend Snippet:

    Article Snippet: Remove the insert of the kinematic fluorescence filter cube (DFM1, Thorlabs) and disassemble it using a hex key ( F and 3G).

    Techniques: Recombinant, Extraction, Software, Fluorescence, Microscopy, Adhesive